Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, K-562 cells, RKO cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26661-1-AP targets EGFL7 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24693 Product name: Recombinant human EGFL7 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 141-273 aa of BC012377 Sequence: CSARRGGCPQRCINTAGSYWCQCWEGHSLSADGTLCVPKGGPPRVAPNPTGVDSAMKEEVQRLQSRVDLLEEKLQLVLAPLHSLASQALEHGLPDPGSLLVHSFQQLGRIDSLSEQISFLEEQLGSCSCKKDS 相同性解析による交差性が予測される生物種 |
| Full Name | EGF-like-domain, multiple 7 |
| Calculated molecular weight | 273 aa, 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC012377 |
| Gene Symbol | EGFL7 |
| Gene ID (NCBI) | 51162 |
| RRID | AB_3669560 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHF1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Epidermal growth factor-like protein 7 (EGFL7, also known as MEGF7, ZNEU1, and VE-STATIN) is a proangiogenic factor that has been widely described as having a vital role in tubulogenesis and regulation of angiogenesis, mainly during embryogenesis organogenesis (PMID: 22160377; 15162510). It also can reduce central nervous system inflammation and may play a role in tumorigenesis (PMID: 29483510; 37490307).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EGFL7 antibody 26661-1-AP | Download protocol |
| WB protocol for EGFL7 antibody 26661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
