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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using EFCAB5 Polyclonal antibody (24296-1-AP)

    WB analysis of HepG2 using 24296-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24296-1-AP (EFCAB5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using EFCAB5 Polyclonal antibody (24296-1-AP)

    WB analysis of L02 using 24296-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24296-1-AP (EFCAB5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EFCAB5 Polyclonal antibody (24296-1-AP)

    IHC staining of human liver cancer using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EFCAB5 Polyclonal antibody (24296-1-AP)

    IHC staining of human liver cancer using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon tissue using EFCAB5 Polyclonal antibody (24296-1-AP)

    IHC staining of human colon using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24296-1-AP (EFCAB5 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using EFCAB5 Polyclonal antibody (24296-1-AP)

    IHC staining of human testis using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using EFCAB5 Polyclonal antibody (24296-1-AP)

    IHC staining of human testis using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using EFCAB5 Polyclonal antibody (24296-1-AP)

    IHC staining of human colon using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using EFCAB5 Polyclonal antibody (24296-1-AP)

    IHC staining of human colon using 24296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24296-1-AP (EFCAB5 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EFCAB5 Polyclonal antibody (24296-1-AP)

    IF Staining of HepG2 using 24296-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EFCAB5 antibody (24296-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

EFCAB5 Polyclonal antibody

EFCAB5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24296-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EF-hand calcium-binding domain-containing protein 5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24296-1-AP targets EFCAB5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19274

Product name: Recombinant human EFCAB5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-342 aa of BC137519

Sequence: MNESASQEELRPAQENRKEDKERKWNLTEVKELHETLQSVPDVPVKEDTNSVVEKAMDEIKSQELNLEGQRKISPGSIKDSKTEASGNIAIRKSAKVIFALDETELKSKPEHTWKKNLFERMEARAQAMQQKIIDKENLKKELEKKAEKKLPRDNLAKEWFNTDSMTLNNTAYLLDKLLPTLVPGVENMLTQVEKKKVLTEADTPSKFDPINYLGEYLIRNNPNYIKDPGMSGYQRLMKEVTEDLKIYVPDTICNRVSKMKENVKQNRKQRESIDKIKVKVANTRKQALQEQFDEWILDPKGMIPKSVIQNVLQEFFQNPDFKLGSHCKQLDITDSTEPRLN

相同性解析による交差性が予測される生物種
Full Name EF-hand calcium binding domain 5
Calculated molecular weight 1503 aa, 173 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC137519
Gene Symbol EFCAB5
Gene ID (NCBI) 374786
RRIDAB_2879479
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA4FU69
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for EFCAB5 antibody 24296-1-APDownload protocol
IHC protocol for EFCAB5 antibody 24296-1-APDownload protocol
WB protocol for EFCAB5 antibody 24296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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