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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EEFSEC Polyclonal antibody (10628-1-AP)

    WB analysis using 10628-1-AP (same clone as 10628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EEFSEC Polyclonal antibody (10628-1-AP)

    WB analysis of HEK-293 using 10628-1-AP (same clone as 10628-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using EEFSEC Polyclonal antibody (10628-1-AP)

    WB analysis of A431 using 10628-1-AP (same clone as 10628-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EEFSEC Polyclonal antibody (10628-1-AP)

    IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using EEFSEC Polyclonal antibody (10628-1-AP)

    IHC staining of human stomach using 10628-1-AP (same clone as 10628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using EEFSEC Polyclonal antibody (10628-1-AP)

    IHC staining of human stomach using 10628-1-AP (same clone as 10628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EEFSEC Polyclonal antibody (10628-1-AP)

    IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

EEFSEC Polyclonal antibody, PBS Only

EEFSEC Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10628-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10628-1-PBS targets EEFSEC in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0980

Product name: Recombinant human EEFSEC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 269-569 aa of BC007933

Sequence: KLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTHKRMVQSP

相同性解析による交差性が予測される生物種
Full Name eukaryotic elongation factor, selenocysteine-tRNA-specific
Calculated molecular weight 63 kDa
Observed molecular weight65 kDa
GenBank accession numberBC007933
Gene Symbol EEFSEC
Gene ID (NCBI) 60678
RRIDAB_2293378
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP57772
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The amino acid selenocysteine (Sec) is incorporated into a small group of proteins that are involved in preventing cellular oxidative stress. The Sec-specific elongation factor eEFec delivers the aminoacylated selenocysteine-tRNA (Sec-tRNASec) to the ribosome and suppresses UGA codons that are upstream of Sec Insertion Sequence (SECIS) elements bound by SECIS binding protein 2 (SBP2)[PMID:22992746]. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon.

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