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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EEF1G Monoclonal antibody (68148-1-Ig)

    WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EEF1G Monoclonal antibody (68148-1-Ig)

    WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EEF1G Monoclonal antibody (68148-1-Ig)

    WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EEF1G Monoclonal antibody (68148-1-Ig)

    WB analysis using 68148-1-Ig (same clone as 68148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using EEF1G Monoclonal antibody (68148-1-Ig)

    WB analysis of MCF-7 using 68148-1-Ig (same clone as 68148-1-PBS)

    WB result of EEF1G antibody (68148-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1G transfected MCF-7 cells. This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EEF1G Monoclonal antibody (68148-1-Ig)

    IF Staining of HeLa using 68148-1-Ig (same clone as 68148-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EEF1G Monoclonal antibody (68148-1-Ig)

    IF Staining of HeLa using 68148-1-Ig (same clone as 68148-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68148-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EEF1G Monoclonal antibody, PBS Only

EEF1G Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68148-1-PBS
Clone No.1G7C6

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

eEF 1B gamma, eEF-1B gamma, EF 1 gamma, EF1G, EF-1-gamma

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68148-1-PBS targets EEF1G in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Reactivity human, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0218

Product name: Recombinant human EEF1G protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-400 aa of BC006520

Sequence: GLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWR

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 gamma
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC006520
Gene Symbol EEF1G
Gene ID (NCBI) 1937
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP26641
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.