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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of Jurkat using 60085-2-Ig (same clone as 60085-2-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of NIH/3T3 using 60085-2-Ig (same clone as 60085-2-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of HeLa using 60085-2-Ig (same clone as 60085-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    WB analysis of RAW 264.7 using 60085-2-Ig (same clone as 60085-2-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using EEF1D Monoclonal antibody (60085-2-Ig)

    IP experiment of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

    IP result of anti-EEF1D (IP:60085-2-Ig, 5ug; Detection:60085-2-Ig 1:1000) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1D Monoclonal antibody (60085-2-Ig)

    IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EEF1D Monoclonal antibody (60085-2-Ig)

    IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 60085-2-Ig

    Affinity and Kinetic Characterization of 60085-2-Ig

    Biolayer interferometry (BLl) kinetic assays of 60085-2-Ig against Human EEF1D were performed. The Affinity Constant is 0.76 nM.

  • Featured Product
  • KD/KO Validated

EEF1D Monoclonal antibody, PBS Only

EEF1D Monoclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 60085-2-PBS
Clone No.3G4C4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

3G4C4, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60085-2-PBS targets EEF1D in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0983

Product name: Recombinant human EEF1D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 347-647 aa of BC007847

Sequence: SHRPGPRSGLSVSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Calculated molecular weight 31 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC007847
Gene Symbol EEF1D
Gene ID (NCBI) 1936
RRIDAB_2096994
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP29692
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

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