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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Endothelin 1 Monoclonal antibody

Endothelin 1 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2C5B3

Cat no : 67008-1-Ig

Synonyms

EDN1, endothelin 1, ET 1 Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1

Validation Data Gallery

Applications:
  • WB analysis of human placenta using 67008-1-Ig

    WB analysis of human placenta using 67008-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67008-1-Ig (EDN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293T using 67008-1-Ig

    FC experiment of HEK-293T using 67008-1-Ig

    1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Endothelin 1 (67008-1-Ig, Clone:2C5B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Tested Applications

Positive WB detected inhuman placenta tissue
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

67008-1-Ig targets Endothelin 1 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Endothelin 1 fusion protein Ag28334 相同性解析による交差性が予測される生物種
Full Name endothelin 1
Calculated molecular weight 212 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC009720
Gene symbol EDN1
Gene ID (NCBI) 1906
RRIDAB_2882325
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).

Protocols

Product Specific Protocols
WB protocol for Endothelin 1 antibody 67008-1-IgDownload protocol
FC protocol for Endothelin 1 antibody 67008-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Rep

Cilostazol regulates the expressions of endothelin-1 and endothelial nitric oxide synthase via activation of the p38 MAPK signaling pathway in HUVECs

Authors - Ying Xue
View Article
humanWB

Hypertens Res

Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation

Authors - Zheng Wang
View Article