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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DPPA2 Polyclonal antibody

DPPA2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 12689-1-AP

Synonyms

DPPA2, ECSA, PESCRG1

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 12689-1-AP

    WB analysis of MCF-7 using 12689-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 12689-1-AP

    WB analysis of HepG2 using 12689-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 12689-1-AP

    WB analysis of MCF-7 using 12689-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 12689-1-AP

    WB analysis of HepG2 using 12689-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12689-1-AP (DPPA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 12689-1-AP

    IF Staining of A431 using 12689-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 12689-1-AP (DPPA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, HepG2 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

12689-1-AP targets DPPA2 in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DPPA2 fusion protein Ag3601 相同性解析による交差性が予測される生物種
Full Name developmental pluripotency associated 2
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC018070
Gene symbol DPPA2
Gene ID (NCBI) 151871
RRIDAB_2277458
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DPPA2 antibody 12689-1-APDownload protocol
IF protocol for DPPA2 antibody 12689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Mol Hum Reprod

Differential expression of the embryo/cancer gene ECSA(DPPA2), the cancer/testis gene BORIS and the pluripotency structural gene OCT4, in human preimplantation development.

Authors - Monk Marilyn M
View Article