Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of A549 using 11521-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11521-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11521-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11521-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 11521-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11521-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 11521-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat pancreas using 11521-1-AP
rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 11521-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 11521-1-AP (ECM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human thyroid cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP (ECM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11521-1-AP (ECM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).
IHC staining of human thyroid cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human thyroid cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human thyroid cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human thyroid cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human pancreas cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human pancreas cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung cancer using 11521-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11521-1-AP (ECM1 antibody) at dilution of 1:50 (under 25x lens).
IF Staining of HepG2 using 11521-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11521-1-AP (ECM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 11521-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ECM1 antibody (11521-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ECM1 Polyclonal antibody

ECM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11521-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

extracellular matrix protein 1, Secretory component p85

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (35)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, L02 cells, HepG2 cells, mouse liver tissue, mouse kidney tissue, MCF-7 cells, mouse pancreas tissue, rat pancreas tissue, A375 cells
Positive IHC detected in	human thyroid cancer tissue, human breast cancer tissue, human lung cancer tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 21 publications below
IHC	See 14 publications below
IF	See 6 publications below
IP	See 1 publications below

Product Information

11521-1-AP targets ECM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2084 Product name: Recombinant human ECM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 241-540 aa of BC023505 Sequence: MSRFCEAEFSVKTRPHWCCTRQGEARFSCFQEEAPQPHYQLRACPSHQPDISSGLELPFPPGVPTLDNIKNICHLRRFRSVPRNLPATDPLQRELLALIQLEREFQRCCRQGNNHTCTWKAWEDTLDKYCDREYAVKTHHHLCCRHPPSPTRDECFARRAPYPNYDRDILTIDISRVTPNLMGHLCGNQRVLTKHKHIPGLIHNMTARCCDLPFPEQACCAEEEKLTFINDLCGPRRNIWRDPALCCYLSPGDEQVNCFNINYLRNVALVSGDTENAKGQGEQGSTGGTNISSTSEPKEE 相同性解析による交差性が予測される生物種
Full Name	extracellular matrix protein 1
Calculated molecular weight	540 aa, 61 kDa
Observed molecular weight	55-61 kDa
GenBank accession number	BC023505
Gene Symbol	ECM1
Gene ID (NCBI)	1893
RRID	AB_2261964
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16610
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis.ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(PMID:21128013) It inhibits MMP9 proteolytic activity. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies(PMID:21497598).

Protocols

Product Specific Protocols
IF protocol for ECM1 antibody 11521-1-AP	Download protocol
IHC protocol for ECM1 antibody 11521-1-AP	Download protocol
WB protocol for ECM1 antibody 11521-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB,IHC	Nat Metab Early-activated extracellular matrix proteins shape the metabolic and spatial dynamics of the kidney fibrotic microenvironment. Authors - Yuan Gui KO Validated View Article
human	WB,IHC,IF	Adv Sci (Weinh) Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer Authors - Xinwen Wang View Article
human	IHC	Cancer Lett Liver metastasis or peritoneal metastasis: single-cell RNA sequencing reveals the organotropism in colorectal cancer is driven by distinct partial-EMT processes Authors - Chengxuan Yu View Article
mouse	WB,IP	Int J Biol Sci Mechanistic study of celastrol-mediated inhibition of proinflammatory activation of macrophages in IgA nephropathy via down-regulating ECM1 Authors - Juanyong Zhao View Article
mouse	WB	Cell Death Discov ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway. Authors - Qi Liu View Article
human, mouse	WB	Acta Pharmacol Sin Nobiletin and polydatin synergistically alleviate triple-negative breast cancer liver metastasis via suppressing ECM1a-mediated fatty acid biosynthesis. Authors - Jue Wang View Article

Validation Data Gallery

WB analysis of A549 using 11521-1-AP

WB analysis of L02 using 11521-1-AP

WB analysis of HepG2 using 11521-1-AP

WB analysis of mouse liver using 11521-1-AP

WB analysis of mouse kidney using 11521-1-AP

WB analysis of MCF-7 using 11521-1-AP

WB analysis of mouse pancreas using 11521-1-AP

WB analysis of rat pancreas using 11521-1-AP

WB analysis of A375 using 11521-1-AP

IHC staining of human thyroid cancer using 11521-1-AP

IHC staining of human thyroid cancer using 11521-1-AP

IHC staining of human pancreas cancer using 11521-1-AP

IHC staining of human thyroid cancer using 11521-1-AP

IHC staining of human thyroid cancer using 11521-1-AP

IHC staining of human thyroid cancer using 11521-1-AP

IHC staining of human thyroid cancer using 11521-1-AP

IHC staining of human breast cancer using 11521-1-AP

IHC staining of human breast cancer using 11521-1-AP

IHC staining of human breast cancer using 11521-1-AP

IHC staining of human breast cancer using 11521-1-AP

IHC staining of human pancreas cancer using 11521-1-AP

IHC staining of human pancreas cancer using 11521-1-AP

IHC staining of human lung cancer using 11521-1-AP

IF Staining of HepG2 using 11521-1-AP

IF Staining of HepG2 using 11521-1-AP

ECM1 Polyclonal antibody

ECM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Early-activated extracellular matrix proteins shape the metabolic and spatial dynamics of the kidney fibrotic microenvironment.

Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer

Liver metastasis or peritoneal metastasis: single-cell RNA sequencing reveals the organotropism in colorectal cancer is driven by distinct partial-EMT processes

Mechanistic study of celastrol-mediated inhibition of proinflammatory activation of macrophages in IgA nephropathy via down-regulating ECM1

ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway.

Nobiletin and polydatin synergistically alleviate triple-negative breast cancer liver metastasis via suppressing ECM1a-mediated fatty acid biosynthesis.

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