Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 11305-1-AP
WB result of ECHS1 antibody (11305-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells.
WB analysis using 11305-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11305-1-AP (ECHS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11305-1-AP
WB result of ECHS1 antibody (11305-1-AP, 1:1200) with si-Control and si-ECHS1 transfected HepG2 cells. .
WB analysis of HeLa using 11305-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11305-1-AP (ECHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 11305-1-AP
IP result of anti-ECHS1 (IP:11305-1-AP, 3ug; Detection:11305-1-AP 1:500) with PC-3 cells lysate 3000ug.
IHC staining of human colon using 11305-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11305-1-AP (ECHS1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 11305-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11305-1-AP (ECHS1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 11305-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11305-1-AP (ECHS1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 11305-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ECHS1 antibody (11305-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ECHS1 Polyclonal antibody

ECHS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11305-1-AP

Publications(31)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:4.2.1.17, ECHM, Enoyl-CoA hydratase 1, Enoyl-CoA hydratase, mitochondrial, mECH

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (31)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, MCF-7 cells, mouse heart, mouse liver, rat liver
Positive IP detected in	PC-3 cells
Positive IHC detected in	human colon tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:600-1:2400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 28 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

11305-1-AP targets ECHS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1842 Product name: Recombinant human ECHS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-290 aa of BC008906 Sequence: MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ 相同性解析による交差性が予測される生物種
Full Name	enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Calculated molecular weight	31 kDa
Observed molecular weight	29-31 kDa
GenBank accession number	BC008906
Gene Symbol	ECHS1
Gene ID (NCBI)	1892
RRID	AB_2262166
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P30084
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

Protocols

Product Specific Protocols
IF protocol for ECHS1 antibody 11305-1-AP	Download protocol
IHC protocol for ECHS1 antibody 11305-1-AP	Download protocol
IP protocol for ECHS1 antibody 11305-1-AP	Download protocol
WB protocol for ECHS1 antibody 11305-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Drug Resist Updat MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance Authors - GuoSheng Wang View Article
human	WB	Cancer Res One-hit effects in cancer: altered proteome of morphologically normal colon crypts in familial adenomatous polyposis. Authors - Yeung Anthony T AT View Article
rat	WB	J Proteome Res Proteomic analysis of left ventricular remodeling in an experimental model of heart failure. Authors - Cieniewski-Bernard Caroline C View Article
human	WB	J Proteome Res Novel Proteomic Biomarker Panel for Prediction of Aggressive Metastatic Hepatocellular Carcinoma Relapse in Surgically Resectable Patients. Authors - Gek San Tan View Article
human	WB	Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. Authors - Liyuan Zhang View Article
mouse	WB	Am J Pathol The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach. Authors - Addabbo Francesco F View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 11305-1-AP

WB analysis using 11305-1-AP

WB analysis of HepG2 using 11305-1-AP

WB analysis of HeLa using 11305-1-AP

IP experiment of PC-3 using 11305-1-AP

IHC staining of human colon using 11305-1-AP

IHC staining of human liver using 11305-1-AP

IHC staining of human liver using 11305-1-AP

IF Staining of HeLa using 11305-1-AP

ECHS1 Polyclonal antibody

ECHS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance

One-hit effects in cancer: altered proteome of morphologically normal colon crypts in familial adenomatous polyposis.

Proteomic analysis of left ventricular remodeling in an experimental model of heart failure.

Novel Proteomic Biomarker Panel for Prediction of Aggressive Metastatic Hepatocellular Carcinoma Relapse in Surgically Resectable Patients.

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach.