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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ECH1 Monoclonal antibody (68139-1-Ig)

    WB analysis using 68139-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using ECH1 Monoclonal antibody (68139-1-Ig)

    WB analysis of HepG2 using 68139-1-Ig

    WB result of ECH1 antibody (68139-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECH1 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ECH1 Monoclonal antibody (68139-1-Ig)

    IHC staining of human liver cancer using 68139-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ECH1 Monoclonal antibody (68139-1-Ig)

    IHC staining of human liver cancer using 68139-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ECH1 Monoclonal antibody (68139-1-Ig)

    IF Staining of HepG2 using 68139-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO Validated

ECH1 Monoclonal antibody

ECH1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68139-1-Ig
Clone No.1C9G5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1C9G5, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, EC:5.3.3.-, HPXEL

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68139-1-Ig targets ECH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag32661

Product name: Recombinant human ECH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-328 aa of BC017408

Sequence: MAAGIVASRRLRDLLTRRLTGSNYPGLSISLRLTGSSAQEAASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQDLVKSVQATTENKELKTVTFSKL

相同性解析による交差性が予測される生物種
Full Name enoyl Coenzyme A hydratase 1, peroxisomal
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 33-36 kDa
GenBank accession numberBC017408
Gene Symbol ECH1
Gene ID (NCBI) 1891
RRIDAB_2923666
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ13011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for ECH1 antibody 68139-1-IgDownload protocol
IHC protocol for ECH1 antibody 68139-1-IgDownload protocol
WB protocol for ECH1 antibody 68139-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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