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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ECH1 Monoclonal antibody

ECH1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C9G5

Cat no : 68139-1-Ig

Synonyms

ECH1, HPXEL

Validation Data Gallery

Applications:
  • WB analysis using 68139-1-Ig

    WB analysis using 68139-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human liver cancer using 68139-1-Ig

    IHC staining of human liver cancer using 68139-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECH1 antibody included
  • IHC staining of human liver cancer using 68139-1-Ig

    IHC staining of human liver cancer using 68139-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECH1 antibody included
  • IF Staining of HepG2 using 68139-1-Ig

    IF Staining of HepG2 using 68139-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ECH1 Ready-To-Use IHC Kit. ECH1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68139-1-Ig targets ECH1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ECH1 fusion protein Ag32661 相同性解析による交差性が予測される生物種
Full Name enoyl Coenzyme A hydratase 1, peroxisomal
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 33-36 kDa
GenBank accession numberBC017408
Gene symbol ECH1
Gene ID (NCBI) 1891
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ECH1 antibody 68139-1-IgDownload protocol
IHC protocol for ECH1 antibody 68139-1-IgDownload protocol
IF protocol for ECH1 antibody 68139-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols