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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ECH1 Polyclonal antibody (11385-1-AP)

    WB analysis of mouse liver using 11385-1-AP (same clone as 11385-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using ECH1 Polyclonal antibody (11385-1-AP)

    WB analysis of mouse heart using 11385-1-AP (same clone as 11385-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using ECH1 Polyclonal antibody (11385-1-AP)

    WB analysis of mouse heart using 11385-1-AP (same clone as 11385-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using ECH1 Polyclonal antibody (11385-1-AP)

    WB analysis of L02 using 11385-1-AP (same clone as 11385-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using ECH1 Polyclonal antibody (11385-1-AP)

    WB analysis of K-562 using 11385-1-AP (same clone as 11385-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ECH1 Polyclonal antibody (11385-1-AP)

    WB analysis of Jurkat using 11385-1-AP (same clone as 11385-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ECH1 Polyclonal antibody (11385-1-AP)

    WB analysis of HepG2 using 11385-1-AP (same clone as 11385-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ECH1 Polyclonal antibody (11385-1-AP)

    IHC staining of human colon using 11385-1-AP (same clone as 11385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11385-1-AP (ECH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

ECH1 Polyclonal antibody, PBS Only

ECH1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11385-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

HPXEL, EC:5.3.3.-, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11385-1-PBS targets ECH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1931

Product name: Recombinant human ECH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-328 aa of BC017408

Sequence: MAAGIVASRRLRDLLTRRLTGSNYPGLSISLRLTGSSAQEAASGVALGEAPDHSYESLRVTSAQKHVLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQDLVKSVQATTENKELKTVTFSKL

相同性解析による交差性が予測される生物種
Full Name enoyl Coenzyme A hydratase 1, peroxisomal
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 30-36 kDa
GenBank accession numberBC017408
Gene Symbol ECH1
Gene ID (NCBI) 1891
RRIDAB_2277810
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13011
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Enoyl coenzyme A hydratase 1 (ECH1), is first recog-nized as a key component in mitochondrial fatty acid β-oxidation, and subsequent studies have demonstrated that it regulates multiple pathophysiological processes (PMID:33813878). The MW of ECH1 is 36 kDa,and can be detected as 30-36 kDa.

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