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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ECE1 Polyclonal antibody

ECE1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27245-1-AP

Synonyms

ECE, ECE 1, ECE1, endothelin converting enzyme 1

Validation Data Gallery

Applications:
  • WB analysis using 27245-1-AP

    WB analysis using 27245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27245-1-AP (ECE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, HUVEC cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ECE1 Ready-To-Use IHC Kit. ECE1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

27245-1-AP targets ECE1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECE1 fusion protein Ag26124 相同性解析による交差性が予測される生物種
Full Name endothelin converting enzyme 1
Observed molecular weight87-110 kDa
GenBank accession numberBC126257
Gene symbol ECE1
Gene ID (NCBI) 1889
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ECE1, Endothelin-converting enzyme 1, also known as ECE. It is located in cell membrane, and it has 4 isoforms. All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney (PMID: 9396733). The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. The calculated molecular weight of ECE1 is 87 kDa, as a result of glycosylation, the apparent molecular mass of ECE1 is approximately 87-110 kDa.

Protocols

Product Specific Protocols
WB protocol for ECE1 antibody 27245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols