Validation Data Gallery

Filter: AllWBIHC

WB analysis using 26088-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 26088-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 26088-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 26088-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 26088-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ECE1 Polyclonal antibody

ECE1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26088-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ECE, ECE 1, ECE1, endothelin converting enzyme 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IF	See 1 publications below

Product Information

26088-1-AP targets ECE1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag23144 Product name: Recombinant human ECE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 335-425 aa of BC126257 Sequence: TAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTK 相同性解析による交差性が予測される生物種
Full Name	endothelin converting enzyme 1
Observed molecular weight	125-135 kDa
GenBank accession number	BC126257
Gene Symbol	ECE1
Gene ID (NCBI)	1889
RRID	AB_2880371
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42892
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for ECE1 antibody 26088-1-AP	Download protocol
WB protocol for ECE1 antibody 26088-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Laryngoscope Investig Otolaryngol Hypoxia-inducible factor-1α contributes to the proliferation of cholesteatoma keratinocytes through regulating endothelin converting enzyme 1 expression Authors - Nie Chen KD Validated View Article
rat	IF	Adv Sci (Weinh) Astrocytic ET-1 System Determines Microglia Phenotype Following Spinal Cord Injury Authors - Bingqiang He View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26088-1-AP

WB analysis of A549 using 26088-1-AP

WB analysis of HeLa using 26088-1-AP

IHC staining of human breast cancer using 26088-1-AP