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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ECD Monoclonal antibody (67179-1-Ig)

    WB analysis using 67179-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67179-1-Ig (ECD antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of human renal cell carcinoma using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of human renal cell carcinoma using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of human stomach cancer using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of human stomach cancer using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of mouse bladder using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of mouse bladder using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of rat colon using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of rat colon using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat bladder tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of rat bladder using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat bladder tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of rat bladder using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ECD Monoclonal antibody

ECD Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67179-1-Ig
Clone No.1D5E8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, JAR cells, NCCIT cells, HEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human stomach cancer tissue, mouse bladder tissue, rat colon tissue, rat bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67179-1-Ig targets ECD in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28674

Product name: Recombinant human ECD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 378-641 aa of BC000721

Sequence: LAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKP

相同性解析による交差性が予測される生物種
Full Name ecdysoneless homolog (Drosophila)
Calculated molecular weight 73 kDa
Observed molecular weight 70-73 kDa
GenBank accession numberBC000721
Gene Symbol ECD
Gene ID (NCBI) 11319
RRIDAB_2882475
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO95905
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for ECD antibody 67179-1-IgDownload protocol
WB protocol for ECD antibody 67179-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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