Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, rat kidney tissue, HeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87174-1-RR targets EBP50/NHERF-1 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag30878 Product name: Recombinant human SLC9A3R1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 260-358 aa of NM_004252 Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | NM_004252 |
| Gene Symbol | SLC9A3R1/EBP50/NHERF-1 |
| Gene ID (NCBI) | 9368 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14745 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EBP50/NHERF-1 antibody 87174-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |