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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    WB analysis using 29771-1-AP (same clone as 29771-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IF Staining of HeLa using 29771-1-AP (same clone as 29771-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IF Staining of A549 using 29771-1-AP (same clone as 29771-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    FC experiment of HepG2 using 29771-1-AP (same clone as 29771-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-HUMAN EBP50/NHERF-1 (29771-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

EBP50/NHERF-1 Polyclonal antibody, PBS Only

EBP50/NHERF-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 29771-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

SLC9A3R1, NHERF-1, EBP50

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29771-1-PBS targets EBP50/NHERF-1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30878

Product name: Recombinant human SLC9A3R1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 260-358 aa of NM_004252

Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
Calculated molecular weight 39 kDa
Observed molecular weight 50 kDa
GenBank accession numberNM_004252
Gene Symbol SLC9A3R1/EBP50/NHERF-1
Gene ID (NCBI) 9368
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14745
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).

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