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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using EBP2 Polyclonal antibody (15973-1-AP)

    WB analysis of MCF-7 using 15973-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EBP2 Polyclonal antibody (15973-1-AP)

    WB analysis of HeLa using 15973-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EBP2 Polyclonal antibody (15973-1-AP)

    WB analysis of HEK-293 using 15973-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using EBP2 Polyclonal antibody (15973-1-AP)

    WB analysis of NIH/3T3 using 15973-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using EBP2 Polyclonal antibody (15973-1-AP)

    WB analysis of HEK-293 using 15973-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using EBP2 Polyclonal antibody (15973-1-AP)

    WB analysis of A549 using 15973-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using EBP2 Polyclonal antibody (15973-1-AP)

    WB analysis of Raji using 15973-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

EBP2 Polyclonal antibody

EBP2 Polyclonal Antibody for WB, ELISA
Cat No. 15973-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EBNA1 binding protein 2, EBNA1BP2, EBP2, NOBP, Nucleolar protein p40, P40

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, A549 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15973-1-AP targets EBP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8760

Product name: Recombinant human EBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-306 aa of BC009175

Sequence: MDTPPLSDSESESDESLVTDRELQDAFSRGLLKPGLNVVLEGPKKAVNDVNGLKQCLAEFKRDLEWVERLDVTLGPVPEIGGSEAPAPQNKDQKAVDPEDDFQREMSFYRQAQAAVLAVLPRLHQLKVPTKRPTDYFAEMAKSDLQMQKIRQKLQTKQAAMERSEKAKQLRALRKYGKKVQTEVLQKRQQEKAHMMNAIKKYQKGFSDKLDFLEGDQKPLAQRKKAGAKGQQMRKGPSAKRRYKNQKFGFGGKKKGSKWNTRESYDDVSSFRAKTAHGRGLKRPGKKGSNKRPGKRTREKMKNRTH

相同性解析による交差性が予測される生物種
Full Name EBNA1 binding protein 2
Calculated molecular weight 306 aa, 35 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC009175
Gene Symbol EBP2
Gene ID (NCBI) 10969
RRIDAB_2878204
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99848
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EBP2 antibody 15973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Oncol

EBP2, a novel NPM-ALK-interacting protein in the nucleolus, contributes to the proliferation of ALCL cells by regulating tumor suppressor p53.

Authors - Yuki Uchihara
  • KD Validated
View Article
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