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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using EAPP Polyclonal antibody (16931-1-AP)

    WB analysis of HEK-293 using 16931-1-AP (same clone as 16931-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16931-1-AP (EAPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16931-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EAPP Polyclonal antibody (16931-1-AP)

    WB analysis of HeLa using 16931-1-AP (same clone as 16931-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16931-1-AP (EAPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16931-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EAPP Polyclonal antibody (16931-1-AP)

    IF Staining of HeLa using 16931-1-AP (same clone as 16931-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16931-1-AP (EAPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16931-1-PBS in a different storage buffer formulation.

EAPP Polyclonal antibody, PBS Only

EAPP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 16931-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16931-1-PBS targets EAPP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10403

Product name: Recombinant human EAPP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-285 aa of BC001245

Sequence: MNRLPDDYDPYAVEEPSDEEPALSSSEDEVDVLLHGTPDQKRKLIRECLTGESESSSEDEFEKEMEAELNSTMKTMEDKLSSLGTGSSSGNGKVATAPTRYYDDIYFDSDSEDEDRAVQVTKKKKKKQHKIPTNDELLYDPEKDNRDQAWVDAQRRGYHGLGPQRSREQQPVPNSDAVLNCPACMTTLCLDCQRHESYKTQYRAMFVMNCSINKEEVLRYKASENRKKRRVHKKMRSNQEDAAEKAETDVEEIYHPVMCTECSTEVAVYDKDEVFHFFNVLASHS

相同性解析による交差性が予測される生物種
Full Name E2F-associated phosphoprotein
Calculated molecular weight 285 aa, 33 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC001245
Gene Symbol EAPP
Gene ID (NCBI) 55837
RRIDAB_2878334
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ56P03
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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