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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using E2F8 Recombinant monoclonal antibody (86645-1-RR)

    WB analysis of HEK-293T using 86645-1-RR

    WB result of E2F8 antibody (86645-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F8 transfected HEK-293T cells.

  • Western Blot (WB) analysis of various lysates using E2F8 Recombinant monoclonal antibody (86645-1-RR)

    WB analysis using 86645-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86645-1-RR (E2F8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using E2F8 Recombinant monoclonal antibody (86645-1-RR)

    WB analysis of HeLa using 86645-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 86645-1-RR (E2F8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using E2F8 Recombinant monoclonal antibody (86645-1-RR)

    IP experiment of HeLa using 86645-1-RR

    IP result of anti-E2F8 (IP:86645-1-RR, 4ug; Detection:86645-1-RR 1:600) with HeLa cells lysate 1120 ug.

  • Affinity and Kinetic Characterization of 86645-1-RR

    Affinity and Kinetic Characterization of 86645-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86645-1-RR against Human E2F8 were performed. The affinity constant is below 1 pM.

  • KD/KO Validated

E2F8 Recombinant monoclonal antibody

E2F8 Uni-rAb® Recombinant Antibody for WB, IP, ELISA
Cat No. 86645-1-RR
Clone No.251510C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

E2F 8, E2F transcription factor 8, E2F-8, Transcription factor E2F8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86645-1-RR targets E2F8 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4216

Product name: Recombinant human E2F8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 78-508 aa of BC028244

Sequence: MAQLAAICKMQLEEQSSESRQKVKVQLARSGPCKPVAPLDPPVNAEMELTAPSLIQPLGMVPLIPSPLSSAVPLILPQAPSGPSYAIYLQPTQAHQSVTPPQGLSPTVCTTHSSKATGSKDSTDATTEKAANDTSKASASTRPGSLLPAPERQGAKSRTREPAGERGSKRASMLEDSGSKKKFKEDLKGLENVSATLFPSGYLIPLTQCSSLGAESILSGKENSSALSPNHRIYSSPIAGVIPVTSSELTAVNFPSFHVTPLKLMVSPTSVAAVPVGNSPALASSHPVPIQNPSSAIVNFTLQHLGLISPNVQLSASPGSGIVPVSPRIESVNVAPENAGTQQGRATNYDSPVPGQSQPNGQSVAVTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSCMDFEGANKTSLGTLFVPQRKLEVSTEDVH

相同性解析による交差性が予測される生物種
Full Name E2F transcription factor 8
Calculated molecular weight 867 aa, 94 kDa
Observed molecular weight105 kDa
GenBank accession numberBC028244
Gene Symbol E2F8
Gene ID (NCBI) 79733
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDA0AVK6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)

Protocols

Product Specific Protocols
IP protocol for E2F8 antibody 86645-1-RRDownload protocol
WB protocol for E2F8 antibody 86645-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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