Validation Data Gallery
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Tested Applications
| Positive WB detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 3 publications below |
| IF | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
24489-1-AP targets E2F7 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E2F7 fusion protein Ag21599 相同性解析による交差性が予測される生物種 |
| Full Name | E2F transcription factor 7 |
| Calculated molecular weight | 911 aa, 100 kDa |
| Observed molecular weight | ~100 kDa |
| GenBank accession number | BC136366 |
| Gene Symbol | E2F7 |
| Gene ID (NCBI) | 144455 |
| RRID | AB_2879569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AV8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
E2F7, also named E2F transcription factor 7, is a 911 amino acid protein belonging to the E2F/DP family. E2F7 localizes in the nucleus and mainly forms homodimers and, to a lesser extent, heterodimers with E2F8. E2F7 as a transcription factor involves in various processes such as angiogenesis, polyploidization of specialized cells, and DNA damage response. E2F7 mainly forms homodimers and to a lesser extent, heterodimers with E2F8. (PMID: 18194653 )
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for E2F7 antibody 24489-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Functional interplay between E2F7 and ribosomal rRNA gene transcription regulates protein synthesis. | ||
Oncotarget The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis. | ||
Cancer Cell Int E2F1/2/7/8 as independent indicators of survival in patients with cervical squamous cell carcinoma.
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Transl Oncol Comprehensive analysis to identify the RP11-478C19.2/ E2F7 axis as a novel biomarker for treatment decisions in clear cell renal cell carcinoma
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