Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 10923-1-AP
WB result of E2F4 antibody (10923-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F4 transfected HeLa cells.
WB analysis of A431 using 10923-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10923-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10923-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10923-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10923-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10923-1-AP
IP result of anti-E2F4 (IP:10923-1-AP, 3ug; Detection:10923-1-AP 1:1000) with mouse brain tissue lysate 3600ug.
IHC staining of human lymphoma using 10923-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 10923-1-AP (E2F4 antibody) at dilution of 1:100 (under 10x lens).

Featured Product
KD/KO Validated

E2F4 Polyclonal antibody

E2F4 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 10923-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

E2F 4, E2F4, Transcription factor E2F4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (16)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, Jurkat cells, HL-60 cells, Raji cells, HeLa cells, NIH/3T3 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 16 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 2 publications below
ChIP	See 2 publications below

Product Information

10923-1-AP targets E2F4 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1365 Product name: Recombinant human E2F4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-413 aa of BC033180 Sequence: MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL 相同性解析による交差性が予測される生物種
Full Name	E2F transcription factor 4, p107/p130-binding
Calculated molecular weight	44 kDa
Observed molecular weight	60-70 kDa
GenBank accession number	BC033180
Gene Symbol	E2F4
Gene ID (NCBI)	1874
RRID	AB_2097096
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16254
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.

Protocols

Product Specific Protocols
IHC protocol for E2F4 antibody 10923-1-AP	Download protocol
IP protocol for E2F4 antibody 10923-1-AP	Download protocol
WB protocol for E2F4 antibody 10923-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Adv Res Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling Authors - Di Fan View Article
mouse	WB,ChIP	Protein Cell Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis. Authors - Yuting Wang View Article
human,mouse	WB	Ecotoxicol Environ Saf Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance Authors - Mengting Xu View Article
human	WB	Oncogene Regulatory networks in mechanotransduction reveal key genes in promoting cancer cell stemness and proliferation. Authors - Wei Huang View Article
mouse	WB,IHC	J Cell Physiol Stable overexpression of p130/E2F4 affects the multipotential abilities of bone-marrow-derived mesenchymal stem cells. Authors - Xiwen Zhang View Article
human	WB	PLoS One Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells. Authors - Valle Blanca L BL View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10923-1-AP

WB analysis of A431 using 10923-1-AP

WB analysis of Jurkat using 10923-1-AP

WB analysis of NIH/3T3 using 10923-1-AP

WB analysis of HL-60 using 10923-1-AP

WB analysis of Raji using 10923-1-AP

IP experiment of mouse brain using 10923-1-AP

IHC staining of human lymphoma using 10923-1-AP

E2F4 Polyclonal antibody

E2F4 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling

Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis.

Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance

Regulatory networks in mechanotransduction reveal key genes in promoting cancer cell stemness and proliferation.

Stable overexpression of p130/E2F4 affects the multipotential abilities of bone-marrow-derived mesenchymal stem cells.

Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.