Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 12171-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 12171-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 12171-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 12171-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 12171-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12171-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12171-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 12171-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 12171-1-AP (E2F1 antibody) at dilution of 1:50 (under 10x lens).

Featured Product
KD/KO Validated

E2F1 Polyclonal antibody

E2F1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12171-1-AP

Publications(65)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Retinoblastoma-associated protein 1, RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (65)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, A431 cells, HeLa cells, mouse brain tissue, NIH/3T3 cells, HepG2 cells, Jurkat cells, LNCaP cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 58 publications below
IHC	See 9 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

12171-1-AP targets E2F1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, artemia sinica
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2813 Product name: Recombinant human E2F1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 88-438 aa of BC050369 Sequence: VKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF 相同性解析による交差性が予測される生物種
Full Name	E2F transcription factor 1
Calculated molecular weight	437 aa, 47 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC050369
Gene Symbol	E2F1
Gene ID (NCBI)	1869
RRID	AB_2096958
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q01094
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 protein. The sumoylated E2F1 is bout 55 kDa.

Protocols

Product Specific Protocols
IHC protocol for E2F1 antibody 12171-1-AP	Download protocol
WB protocol for E2F1 antibody 12171-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma. Authors - Dan Su View Article
mouse	IHC	J Clin Invest TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. Authors - Zheng Hu View Article
	WB	J Exp Clin Cancer Res ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma. Authors - Mengzhao Zhang View Article
human	WB	Adv Sci (Weinh) Novel Evolutionarily Conserved Oncogene COA4 is Driven by KRAS Mutant and Promotes Cancer Metastasis Through Dual Mitochondrial Metabolism-Dependent and -Independent Mechanisms. Authors - Xingzhao Ji View Article
human	WB	Cell Death Dis miR-148a-3p represses proliferation and EMT by establishing regulatory circuits between ERBB3/AKT2/c-myc and DNMT1 in bladder cancer. Authors - Xiao Wang View Article
human	WB	Cell Death Dis PMID: 29358577 Authors - Zhi-Rui Lin View Article

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Validation Data Gallery

WB analysis of HeLa using 12171-1-AP

WB analysis using 12171-1-AP

WB analysis of A431 using 12171-1-AP

WB analysis of NIH/3T3 using 12171-1-AP

WB analysis of A431 using 12171-1-AP

WB analysis of HeLa using 12171-1-AP

WB analysis of mouse brain using 12171-1-AP

IHC staining of human breast cancer using 12171-1-AP

E2F1 Polyclonal antibody

E2F1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.

ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma.

Novel Evolutionarily Conserved Oncogene COA4 is Driven by KRAS Mutant and Promotes Cancer Metastasis Through Dual Mitochondrial Metabolism-Dependent and -Independent Mechanisms.

miR-148a-3p represses proliferation and EMT by establishing regulatory circuits between ERBB3/AKT2/c-myc and DNMT1 in bladder cancer.

PMID: 29358577

Able^™

Validation Data Gallery

WB analysis of HeLa using 12171-1-AP

WB analysis using 12171-1-AP

WB analysis of A431 using 12171-1-AP

WB analysis of NIH/3T3 using 12171-1-AP

WB analysis of A431 using 12171-1-AP

WB analysis of HeLa using 12171-1-AP

WB analysis of mouse brain using 12171-1-AP

IHC staining of human breast cancer using 12171-1-AP

E2F1 Polyclonal antibody

E2F1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.

ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma.

Novel Evolutionarily Conserved Oncogene COA4 is Driven by KRAS Mutant and Promotes Cancer Metastasis Through Dual Mitochondrial Metabolism-Dependent and -Independent Mechanisms.

miR-148a-3p represses proliferation and EMT by establishing regulatory circuits between ERBB3/AKT2/c-myc and DNMT1 in bladder cancer.

PMID: 29358577

Able™

Able^™