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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Dysbindin Polyclonal antibody (11132-1-AP)

    WB analysis of mouse brain using 11132-1-AP (same clone as 11132-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11132-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using Dysbindin Polyclonal antibody (11132-1-AP)

    WB analysis of mouse testis using 11132-1-AP (same clone as 11132-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11132-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using Dysbindin Polyclonal antibody (11132-1-AP)

    WB analysis of human testis using 11132-1-AP (same clone as 11132-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 11132-1-AP (Dysbindin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Dysbindin Polyclonal antibody (11132-1-AP)

    IHC staining of mouse brain using 11132-1-AP (same clone as 11132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11132-1-AP (Dysbindin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Dysbindin Polyclonal antibody (11132-1-AP)

    IHC staining of mouse brain using 11132-1-AP (same clone as 11132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11132-1-AP (Dysbindin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11132-1-PBS in a different storage buffer formulation.

Dysbindin Polyclonal antibody, PBS Only

Dysbindin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11132-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11132-1-PBS targets Dysbindin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1613

Product name: Recombinant human DTNBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC011912

Sequence: MLETLRERLLSVQQDFTSGLKTLSDKSREAKVKSKPRTVPFLPKYSAGLELLSRYEDTWAALHRRAKDCASAGELVDSEVVMLSAHWEKKKTSLVELQEQLQQLPALIADLESMTANLTHLEASFEEVENNLLHLEDLCGQCELERCKHMQSQQLENYKKNKRKELETFKAELDAEHAQKVLEMEHTQQMKLKERQKFFEEAFQQDMEQYLSTGYLQIAERREPIGSMSSMEVNVDMLEQMDLMDISDQEALDVFLNSGGEENTVLSPALGPESSTCQNEITLQVPNPSELRAKPPSSSSTCTDSATRDISEGGESPVVQSDEEEVQVDTALATSHTDREATPDGGEDSDS

相同性解析による交差性が予測される生物種
Full Name dystrobrevin binding protein 1
Calculated molecular weight 351 aa, 39 kDa
Observed molecular weight 48 kDa, 36 kDa
GenBank accession numberBC011912
Gene Symbol Dysbindin
Gene ID (NCBI) 84062
RRIDAB_11182503
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EV8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Dysbindin, encoded by DTNBP1 (dystrobrevin binding protein 1), is a synaptic protein which is expressed in diverse neuronal populations throughout the brain. Genetic variation in DTNBP1 is associated with schizophrenia. Reduced expression of dysbindin has been reported in the dorsolateral prefrontal cortex (DLPFC) of schizophrenia cases. Several isoforms of dysbindin exist: 48-50 kDa of dysbindin-1A, 35-38 kDa of dysbindin-1B, and 30-32 kDa of dysbindin-1C. This antibody recognizes all of isoforms of dysbindin. (19617633)

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