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Tested Applications
| Positive WB detected in | THP-1 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells |
| Positive Dot Blot detected in | peptide |
This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82859-1-RR targets Di-Methyl-Histone H4 (Arg3) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 1, H4a |
| Observed molecular weight | 13 kDa |
| GenBank accession number | BC069654 |
| Gene Symbol | HIST1H4A |
| Gene ID (NCBI) | 8359 |
| RRID | AB_3670583 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62805 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Arginine dimethylation is an important post-translational modification of proteins. Based on the positions of the two methyl groups on the guanidino group of arginine, it can be divided into symmetric dimethylation (SDMA) and asymmetric dimethylation (ADMA). Symmetric dimethylation is crucial for embryonic development and cell differentiation and is often associated with transcriptional repression. This antibody specifically recognizes symmetric dimethyl-arginine (SDMA, Rme2s).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Di-Methyl-Histone H4 (Arg3) antibody 82859-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |