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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Di-Methyl-Histone H3 (Lys56) Recombinant monoclona (82828-12-RR)

    WB analysis using 82828-12-RR (same clone as 82828-12-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82828-12-RR (HIST1H3A(H3K56me2) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Di-Methyl-Histone H3 (Lys56) Recombinant monoclona (82828-12-RR)

    IF Staining of NIH/3T3 using 82828-12-RR (same clone as 82828-12-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Lys56) antibody (82828-12-RR, Clone: 243027A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Di-Methyl-Histone H3 (Lys56) Recombinant monoclona (82828-12-RR)

    IF Staining of HepG2 using 82828-12-RR (same clone as 82828-12-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Di-Methyl-Histone H3 (Lys56) antibody (82828-12-RR, Clone: 243027A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

  • Dot Blot experiment using Di-Methyl-Histone H3 (Lys56) Recombinant monoclona (82828-12-RR)

    Dot Blot experiment using 82828-12-RR (same clone as 82828-12-PBS)

    Dot blot analysis was used to confirm the specificity of 82828-12-RR (Di-Methyl-Histone H3 (Lys56) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82828-12-PBS in a different storage buffer formulation.

Di-Methyl-Histone H3 (Lys56) Recombinant monoclonal antibody, PBS Only

Di-Methyl-Histone H3 (Lys56) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA
Cat No. 82828-12-PBS
Clone No.243027A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA

Synonyms

Di Methyl Histone H3, Di-Methyl Histone H3 (Lys56), Di-Methyl Histone H3 Lys56, Di-Methyl Histone-H3 Lys56, Di-Methyl-Histone H3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82828-12-PBS targets Di-Methyl-Histone H3 (Lys56) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15-18 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).

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