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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Di-Methyl-Histone H3 (Lys27) Recombinant monoclona (86890-2-RR)

    WB analysis using 86890-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86890-2-RR (Di-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Di-Methyl-Histone H3 (Lys27) Recombinant monoclona (86890-2-RR)

    WB analysis using 86890-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86890-2-RR (Di-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Di-Methyl-Histone H3 (Lys27) Recombinant monoclona (86890-2-RR)

    IF Staining of HeLa using 86890-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Di-Methyl-Histone H3 (Lys27) antibody (86890-2-RR, Clone: 251846E11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of / using Di-Methyl-Histone H3 (Lys27) Recombinant monoclona (86890-2-RR)

    Dot Blot experiment of / using 86890-2-RR

    Dot blot analysis was used to confirm the specificity of 86890-2-RR Di-Methyl-Histone H3 (Lys27) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

Di-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody

Di-Methyl-Histone H3 (Lys27) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA
Cat No. 86890-2-RR
Clone No.251846E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Dot Blot, ELISA

Synonyms

DiMethylHistone H3, Di-Methyl-Histone H3, Di-MethylHistone H3 (Lys27), Di-MethylHistone H3 Lys27, Di-Methyl-Histone-H3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293T cells, K-562 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected in/ /

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
DOT BLOTDOT BLOT : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86890-2-RR targets Di-Methyl-Histone H3 (Lys27) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight17 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3745190
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for Di-Methyl-Histone H3 (Lys27) antibody 86890-2-RRDownload protocol
WB protocol for Di-Methyl-Histone H3 (Lys27) antibody 86890-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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