• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human placenta tissue using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    IHC staining of human placenta using 84702-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    IHC staining of rat liver using 84702-1-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    IHC staining of mouse liver using 84702-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    IF Staining of HeLa using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    IF Staining of NIH/3T3 using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    IF Staining of C6 using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    FC experiment of HeLa using 84702-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Dot Blot experiment of peptide using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    Dot Blot experiment of peptide using 84702-1-RR

    Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa cells using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    ChIP experiment of HeLa using 84702-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di-Methyl-Histone H3 (Arg2) (84702-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

Di-Methyl-Histone H3 (Arg2) Recombinant monoclonal antibody

Di-Methyl-Histone H3 (Arg2) Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR
Cat No. 84702-1-RR
Clone No.241896D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR

Synonyms

Di Methyl Histone H3, Di-Methyl Histone H3 (Arg2), Di-Methyl Histone H3 Arg2, Di-Methyl Histone-H3 Arg2, Di-Methyl-Histone H3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inrat liver tissue, human placenta tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells, C6 cells
Positive FC (Intra) detected inHeLa cells
Positive Dot Blot detected inpeptide
Positive ChIP-qPCR detected inHeLa cells

This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84702-1-RR targets Di-Methyl-Histone H3 (Arg2) in IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3672118
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for Di-Methyl-Histone H3 (Arg2) antibody 84702-1-RRDownload protocol
IHC protocol for Di-Methyl-Histone H3 (Arg2) antibody 84702-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...