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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Destrin Polyclonal antibody (10962-2-AP)

    WB analysis using 10962-2-AP (same clone as 10962-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10962-2-AP (Destrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10962-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Destrin Polyclonal antibody (10962-2-AP)

    IHC staining of human skin cancer using 10962-2-AP (same clone as 10962-2-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10962-2-AP (Destrin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10962-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Destrin Polyclonal antibody (10962-2-AP)

    IF Staining of MCF-7 using 10962-2-AP (same clone as 10962-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Destrin antibody (10962-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10962-2-PBS in a different storage buffer formulation.

Destrin Polyclonal antibody, PBS Only

Destrin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10962-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DSTN, DSN, ADF, Actin-depolymerizing factor, ACTDP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10962-2-PBS targets Destrin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1402

Product name: Recombinant human DSTN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-165 aa of BC009477

Sequence: MASGVQVADEVCRIFYDMKVRKCSTPEEIKKRKKAVIFCLSADKKCIIVEEGKEILVGDVGVTITDPFKHFVGMLPEKDCRYALYDASFETKESRKEELMFFLWAPELAPLKSKMIYASSKDAIKKKFQGIKHECQANGPEDLNRACIAEKLGGSLIVAFEGCPV

相同性解析による交差性が予測される生物種
Full Name destrin (actin depolymerizing factor)
Calculated molecular weight 19 kDa
Observed molecular weight18 kDa
GenBank accession numberBC009477
Gene Symbol Destrin
Gene ID (NCBI) 11034
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP60981
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.

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