Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse skin tissue, mouse uterus tissue, mouse heart tissue, mouse colon tissue, rat colon tissue, rat uterus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85737-4-RR targets Decorin in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Decorin fusion protein Eg2955 相同性解析による交差性が予測される生物種 |
| Full Name | decorin |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 45-100 kDa |
| GenBank accession number | NM_001190451.2 |
| Gene Symbol | Dcn |
| Gene ID (NCBI) | 13179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P28654 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Decorin antibody 85737-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |