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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DZIP1 Polyclonal antibody (13779-1-AP)

    WB analysis of HEK-293 using 13779-1-AP (same clone as 13779-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using DZIP1 Polyclonal antibody (13779-1-AP)

    WB analysis of human testis using 13779-1-AP (same clone as 13779-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using DZIP1 Polyclonal antibody (13779-1-AP)

    WB analysis of mouse skeletal muscle using 13779-1-AP (same clone as 13779-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using DZIP1 Polyclonal antibody (13779-1-AP)

    WB analysis of human brain using 13779-1-AP (same clone as 13779-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using DZIP1 Polyclonal antibody (13779-1-AP)

    WB analysis of PC-3 using 13779-1-AP (same clone as 13779-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13779-1-AP (DZIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using DZIP1 Polyclonal antibody (13779-1-AP)

    IP experiment of HEK-293 using 13779-1-AP (same clone as 13779-1-PBS)

    IP result of anti-DZIP1 (IP:13779-1-AP, 4ug; Detection:13779-1-AP 1:300) with HEK-293 cells lysate 880ug. This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using DZIP1 Polyclonal antibody (13779-1-AP)

    IHC staining of human oesophagus cancer using 13779-1-AP (same clone as 13779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13779-1-AP (DZIP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DZIP1 Polyclonal antibody (13779-1-AP)

    IHC staining of mouse testis using 13779-1-AP (same clone as 13779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13779-1-AP (DZIP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using DZIP1 Polyclonal antibody (13779-1-AP)

    IF Staining of PC-3 using 13779-1-AP (same clone as 13779-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13779-1-AP (DZIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13779-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DZIP1 Polyclonal antibody, PBS Only

DZIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13779-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DZIP, DAZ-interacting zinc finger protein 1, DAZ-interacting protein 1/2, DAZ interacting protein 1/2, DAZ interacting protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13779-1-PBS targets DZIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4772

Product name: Recombinant human DZIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 496-867 aa of BC041804

Sequence: FSSHILEPIEELSEEEKGRENEQKLNNNKMHLRKALKSNSSLTKGLRTMVEQNLMEKLETLGINADIRGISSDQLHRVLKSVESERHKQEREIPNFHQIREFLEHQVSCKIEEKALLSSDQCSVSQMDTLSTGEVPKMIQLPSKNRQLIRQKAVSTDRTSVPKIKKNVMEDPFPRKSSTITTPPFSSEEEQEDDDLIRAYASPGPLPVPPPQNKGSFGKNTVKSDADGTEGSEIEDTDDSPKPAGVAVKTPTEKVEKMFPHRKNVNKPVGGTNVPEMFIKKEELQELKCADVEDEDWDISSLEEEISLGKKSGKEQKEPPPAKNEPHFAHVLNAWGAFNPKGPKGEGLQENESSTLKSSLVTVTDWSDTSDV

相同性解析による交差性が予測される生物種
Full Name DAZ interacting protein 1
Calculated molecular weight 867 aa, 98 kDa
Observed molecular weight 100-113 kDa
GenBank accession numberBC041804
Gene Symbol DZIP1
Gene ID (NCBI) 22873
RRIDAB_2096927
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YF9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DZIP1, also named as DZIP, DZIP2 and KIAA0996, belongs to the DZIP C2H2-type zinc-finger protein family. DZIP1 has been reported to be associated with centrosomes and ciliogenesis in cells (PMID: 32051257). DZIP1 may participate in spermatogenesis via its interaction with DAZ1, and play a role in primary formation (PMID: 15081113, 19852954). DZIP1 has 3 isoforms with the molecular mass of 31, 97 and 99 kDa. It may be a potential therapeutic target of oral squamous carcinoma (PMID: 31450126).

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