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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DYSFIP1 Polyclonal antibody (18208-1-AP)

    WB analysis using 18208-1-AP (same clone as 18208-1-PBS)

    Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18208-1-AP (DYSFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using DYSFIP1 Polyclonal antibody (18208-1-AP)

    IF Staining of THP-1 using 18208-1-AP (same clone as 18208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using DYSFIP1 antibody (18208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18208-1-PBS in a different storage buffer formulation.

DYSFIP1 Polyclonal antibody, PBS Only

DYSFIP1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 18208-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Toonin, Protein phosphatase 1 regulatory subunit 27, PPP1R27, Dysferlin-interacting protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18208-1-PBS targets DYSFIP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12839

Product name: Recombinant human DYSFIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-154 aa of BC093707

Sequence: MPSRTARYARYSPRQRRRRMLADRSVRFPNDVLFLDHIRQGDLEQVGRFIRTRKVSLATIHPSGLAALHEAVLSGNLECVKLLVKYGADIHQRDEAGWTPLHIACSDGYPDIARYLISLGADRDATNDDGDLPSDLIDPDYKELVELFKGTTMD

相同性解析による交差性が予測される生物種
Full Name dysferlin interacting protein 1
Calculated molecular weight 154 aa, 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC093707
Gene Symbol DYSFIP1
Gene ID (NCBI) 116729
RRIDAB_3669295
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WC6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DYSFIP1 is also known as PPP1R27. DYSFIP1 inhibits phosphatase activity of protein phosphatase 1 (PP1) complexes. The molecular weight of DYSFIP1 is 17 kDa.

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