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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DYNC1H1 Polyclonal antibody (12345-1-AP)

    WB analysis using 12345-1-AP (same clone as 12345-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DYNC1H1 Polyclonal antibody (12345-1-AP)

    WB analysis using 12345-1-AP (same clone as 12345-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using DYNC1H1 Polyclonal antibody (12345-1-AP)

    IP experiment of HeLa using 12345-1-AP (same clone as 12345-1-PBS)

    IP result of anti-DYNC1H1 (IP:12345-1-AP, 4ug; Detection:12345-1-AP 1:5000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DYNC1H1 Polyclonal antibody (12345-1-AP)

    IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1  antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DYNC1H1 Polyclonal antibody (12345-1-AP)

    IHC staining of mouse brain using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1  antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using DYNC1H1 Polyclonal antibody (12345-1-AP)

    IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using DYNC1H1 Polyclonal antibody (12345-1-AP)

    IHC staining of human colon using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DYNC1H1 Polyclonal antibody (12345-1-AP)

    IHC staining of human breast cancer using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12345-1-AP (DYNC1H1  antibody at dilution of 1:50. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using DYNC1H1 Polyclonal antibody (12345-1-AP)

    IHC staining of human testis using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1  antibody at dilution of 1:50. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using DYNC1H1 Polyclonal antibody (12345-1-AP)

    IHC staining of human testis using 12345-1-AP (same clone as 12345-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1  antibody at dilution of 1:50. This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DYNC1H1 Polyclonal antibody (12345-1-AP)

    IF Staining of MCF-7 using 12345-1-AP (same clone as 12345-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DYNC1H1 antibody (12345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12345-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DYNC1H1 Polyclonal antibody, PBS Only

DYNC1H1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12345-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DYNC1H 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12345-1-PBS targets DYNC1H1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2999

Product name: Recombinant human KIAA0325 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 590-989 aa of BC021297

Sequence: MAQSIYGGRVDNEFDQRLLNTFLERLFTTRSFDSEFKLACKVDGHKDIQMPDGIRREEFVQWVELLPDTQTPSWLGLPNNAERVLLTTQGVDMISKMLKMQMLEDEDDLAYAETEKKTRTDSTSDGRPAWMRTLHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTLINELVKGILPRSWSHYTVPAGMTVIQWVSDFSERIKQLQNISLAAASGGAKELKNIHVCLGGLFVPEAYITATRQYVAQANSWSLEELCLEVNVTTSQGATLDACSFGVTGLKLQGATCNNNKLSLSNAISTALPLTQLRWVKQTNTEKKASVVTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE

相同性解析による交差性が予測される生物種
Full Name dynein, cytoplasmic 1, heavy chain 1
Calculated molecular weight 4646 aa, 532 kDa
Observed molecular weight 532 kDa
GenBank accession numberBC021297
Gene Symbol DYNC1H1
Gene ID (NCBI) 1778
RRIDAB_2261765
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14204
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Dyneins are a group of microtubule-activated ATPases that convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein comprises 2 heavy chain polypeptides and several intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients.

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