Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32123-1-AP targets DYNAP in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37125 Product name: Recombinant human C18orf26 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 135-210 aa of NM_001307955 Sequence: LVNNKGSANSSIVIQLSTNDGECVTVKPGTPSPACPPTMTTTSTVPASTATESTTSTATAATTSTEPITVAPTDHL 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 18 open reading frame 26 |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | NM_001307955 |
| Gene Symbol | C18orf26 |
| Gene ID (NCBI) | 284254 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N1N2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The dynactin-associated protein (DYNAP), also known as C18orf26, is a transmembrane protein that localises to the Golgi apparatus and the plasma membrane in a microtubule-dependent manner. This protein is expressed in half of all human cancer cell lines. DYNAP expression activates Akt (also known as protein kinase B) by promoting Ser473 phosphorylation, which is required for full activation (PMID: 20978158). In SDS-PAGE, DYNAP migrates anomalously at ~45 kDa, which is much larger than the expected 22.5 kDa based on its amino acid composition. The T/S cluster region is responsible for this (PMID: 35022995).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNAP antibody 32123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |