• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DVL2 Monoclonal antibody

DVL2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F2D2

Cat no : 67105-1-Ig

Synonyms

Dishevelled 2, DSH homolog 2, DVL2

Validation Data Gallery

Applications:
  • WB analysis using 67105-1-Ig

    WB analysis using 67105-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 67105-1-Ig

    WB analysis of A431 using 67105-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67105-1-Ig

    WB analysis using 67105-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • WB analysis of T-47D using 67105-1-Ig

    WB analysis of T-47D using 67105-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 67105-1-Ig

    IHC staining of mouse colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of mouse colon using 67105-1-Ig

    IHC staining of mouse colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of rat colon using 67105-1-Ig

    IHC staining of rat colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of rat colon using 67105-1-Ig

    IHC staining of rat colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human colon cancer using 67105-1-Ig

    IHC staining of human colon cancer using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human colon cancer using 67105-1-Ig

    IHC staining of human colon cancer using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human prostate cancer using 67105-1-Ig

    IHC staining of human prostate cancer using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human prostate cancer using 67105-1-Ig

    IHC staining of human prostate cancer using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human gliomas using 67105-1-Ig

    IHC staining of human gliomas using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of human gliomas using 67105-1-Ig

    IHC staining of human gliomas using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included

Tested Applications

Positive WB detected inMCF-7 cells, A431 cells, T-47D cells, THP-1 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse colon tissue, human colon cancer tissue, human gliomas tissue, human prostate cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DVL2 Ready-To-Use IHC Kit. DVL2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

67105-1-Ig targets DVL2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DVL2 fusion protein Ag28634 相同性解析による交差性が予測される生物種
Full Name dishevelled, dsh homolog 2 (Drosophila)
Calculated molecular weight 736 aa, 79 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC014844
Gene symbol DVL2
Gene ID (NCBI) 1856
RRIDAB_2882409
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DVL2 antibody 67105-1-IgDownload protocol
IHC protocol for DVL2 antibody 67105-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols