Validation Data Gallery

Filter: AllWBIHC

WB analysis using 67105-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 67105-1-Ig
A431 cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 67105-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of T-47D using 67105-1-Ig
T-47D cells were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of mouse colon using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 67105-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DVL2 Monoclonal antibody

DVL2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67105-1-Ig

Clone No.2F2D2

Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Dishevelled 2, 2F2D2, Dishevelled-2, DSH homolog 2, Segment polarity protein dishevelled homolog DVL-2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, A431 cells, T-47D cells, THP-1 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected in	mouse colon tissue, human colon cancer tissue, human gliomas tissue, human prostate cancer tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IP	See 1 publications below

Product Information

67105-1-Ig targets DVL2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28634 Product name: Recombinant human DVL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-340 aa of BC014844 Sequence: MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDI 相同性解析による交差性が予測される生物種
Full Name	dishevelled, dsh homolog 2 (Drosophila)
Calculated molecular weight	736 aa, 79 kDa
Observed molecular weight	90 kDa
GenBank accession number	BC014844
Gene Symbol	DVL2
Gene ID (NCBI)	1856
RRID	AB_2882409
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O14641
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for DVL2 antibody 67105-1-Ig	Download protocol
WB protocol for DVL2 antibody 67105-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Int J Biol Sci CYR61 Acts as an Intracellular Microtubule-Associated Protein and Coordinates Mitotic Progression via PLK1-FBW7 Pathway Authors - Kaishun Hu View Article
rat	WB	Biochim Biophys Acta Mol Cell Res Wnt/β-catenin signaling inhibits oxidative stress-induced ferroptosis to improve interstitial cystitis/bladder pain syndrome by reducing NF-κB Authors - Weilin Fang View Article
human	WB	bioRxiv Wnt specifically induces FZD5/8 endocytosis and degradation and the involvement of RSPO-ZNRF3/RNF43 and DVL Authors - Dong Luo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 67105-1-Ig

WB analysis of A431 using 67105-1-Ig

WB analysis using 67105-1-Ig

WB analysis of T-47D using 67105-1-Ig

IHC staining of mouse colon using 67105-1-Ig

IHC staining of mouse colon using 67105-1-Ig

IHC staining of rat colon using 67105-1-Ig

IHC staining of human colon cancer using 67105-1-Ig