Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HepG2 cells using 12037-1-AP
WB result of DVL2 antibody (12037-1-AP, 1:1000) with siDVL2 and si-control transfected cells.
WB analysis using 12037-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12037-1-AP
WB result of DVL2 antibody (12037-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DVL2 transfected HEK-293 cells.
WB analysis of mouse thymus using 12037-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 12037-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12037-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
IP experiment of MCF-7 using 12037-1-AP
IP result of anti-DVL2 (IP:12037-1-AP, 4ug; Detection:12037-1-AP 1:4000) with MCF-7 cells lysate 640 ug.
IHC staining of human lung cancer using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 12037-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12037-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DVL2 antibody (12037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

DVL2 Polyclonal antibody

DVL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12037-1-AP

Publications(37)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Dishevelled 2, Dishevelled-2, DSH homolog 2, Segment polarity protein dishevelled homolog DVL-2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (37)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, mouse thymus tissue, MDA-MB-231 cells, NIH/3T3 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human lung cancer tissue, human gliomas tissue, human prostate cancer tissue, mouse colon tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 31 publications below
IHC	See 5 publications below
IF	See 9 publications below
IP	See 3 publications below
CoIP	See 2 publications below

Product Information

12037-1-AP targets DVL2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2666 Product name: Recombinant human DVL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC014844 Sequence: MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDI 相同性解析による交差性が予測される生物種
Full Name	dishevelled, dsh homolog 2 (Drosophila)
Calculated molecular weight	736 aa, 79 kDa
Observed molecular weight	90-95 kDa
GenBank accession number	BC014844
Gene Symbol	DVL2
Gene ID (NCBI)	1856
RRID	AB_2093330
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14641
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DVL2 is member of the dishevelled (dsh) protein family. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein in a variety of cell lines and species. DVL2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes.

Protocols

Product Specific Protocols
IF protocol for DVL2 antibody 12037-1-AP	Download protocol
IHC protocol for DVL2 antibody 12037-1-AP	Download protocol
IP protocol for DVL2 antibody 12037-1-AP	Download protocol
WB protocol for DVL2 antibody 12037-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer A multidimensional recommendation framework for identifying biological targets to aid the diagnosis and treatment of liver metastasis in patients with colorectal cancer Authors - Feng Qi View Article
pig	WB	J Nanobiotechnology Maternal fiber-rich diet promotes early-life intestinal development in offspring through milk-derived extracellular vesicles carrying miR-146a-5p Authors - Dongdong Lu View Article
human	WB	Cell Death Dis YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury. Authors - Feihong Deng View Article
rat	WB	Phytomedicine SiNiSan alleviates liver injury by promoting hepatic stem cell differentiation via Wnt/β-catenin signaling pathway. Authors - Weidong Xu View Article
human	WB	Acta Pharmacol Sin CYP2A6 suppresses hepatocellular carcinoma via inhibiting SRC/Wnt/β-Catenin pathway Authors - Yi-Fan Liu View Article
human	WB	Cell Death Discov MYH9 is crucial for stem cell-like properties in non-small cell lung cancer by activating mTOR signaling. Authors - Meng Chen View Article

Validation Data Gallery

WB analysis of HepG2 cells using 12037-1-AP

WB analysis using 12037-1-AP

WB analysis of HEK-293 using 12037-1-AP

WB analysis of mouse thymus using 12037-1-AP

WB analysis of MDA-MB-453s using 12037-1-AP

WB analysis of MCF-7 using 12037-1-AP

IP experiment of MCF-7 using 12037-1-AP

IHC staining of human lung cancer using 12037-1-AP

IHC staining of human lung cancer using 12037-1-AP

IHC staining of human gliomas using 12037-1-AP

IHC staining of human gliomas using 12037-1-AP

IHC staining of human prostate cancer using 12037-1-AP

IHC staining of human prostate cancer using 12037-1-AP

IHC staining of mouse colon using 12037-1-AP

IHC staining of mouse colon using 12037-1-AP

IHC staining of rat colon using 12037-1-AP

IHC staining of rat colon using 12037-1-AP

IHC staining of human gliomas using 12037-1-AP

IHC staining of human gliomas using 12037-1-AP

IF Staining of HepG2 using 12037-1-AP

DVL2 Polyclonal antibody

DVL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A multidimensional recommendation framework for identifying biological targets to aid the diagnosis and treatment of liver metastasis in patients with colorectal cancer

Maternal fiber-rich diet promotes early-life intestinal development in offspring through milk-derived extracellular vesicles carrying miR-146a-5p

YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury.

SiNiSan alleviates liver injury by promoting hepatic stem cell differentiation via Wnt/β-catenin signaling pathway.

CYP2A6 suppresses hepatocellular carcinoma via inhibiting SRC/Wnt/β-Catenin pathway

MYH9 is crucial for stem cell-like properties in non-small cell lung cancer by activating mTOR signaling.

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