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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DUSP6 Recombinant monoclonal antibody (83356-5-RR)

    WB analysis using 83356-5-RR (same clone as 83356-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83356-5-RR (DUSP6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83356-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DUSP6 Recombinant monoclonal antibody (83356-5-RR)

    FC experiment of HeLa using 83356-5-RR (same clone as 83356-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83356-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-12 cells using DUSP6 Recombinant monoclonal antibody (83356-5-RR)

    FC experiment of PC-12 using 83356-5-RR (same clone as 83356-5-PBS)

    1x10^6 PC-12 cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83356-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83356-5-RR

    Affinity and Kinetic Characterization of 83356-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83356-5-RR against Human DUSP6 were performed. The affinity constant is 3.38 nM.

DUSP6 Recombinant monoclonal antibody, PBS Only

DUSP6 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83356-5-PBS
Clone No.240295C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

240295C8, dual specificity phosphatase 6, EC:3.1.3.16, MAP kinase phosphatase 3, Mitogen-activated protein kinase phosphatase 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83356-5-PBS targets DUSP6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag23474

Product name: Recombinant human DUSP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 353-381 aa of BC003562

Sequence: CDNRVPAQQLYFTTPSNQNVYQVDSLQST

相同性解析による交差性が予測される生物種
Full Name dual specificity phosphatase 6
Calculated molecular weight42 kDa
Observed molecular weight42-45 kDa
GenBank accession numberBC003562
Gene Symbol DUSP6
Gene ID (NCBI) 1848
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ16828
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).

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