Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, rat brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
28284-1-AP targets DUSP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28575 Product name: Recombinant human DUSP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-185 aa of BC002682 Sequence: MSGSFELSVQDLNDLLSDGSGCYSLPSQPCNEVTPRIYVGNASVAQDIPKLQKLGITHVLNAAEGRSFMHVNTNANFYKDSGITYLGIKANDTQEFNLSAYFERAADFIDQALAQKNGRVLVHCREGYSRSPTLVIAYLMMRQKMDVKSALSIVRQNREIGPNDGFLAQLCQLNDRLAKEGKLKP 相同性解析による交差性が予測される生物種 |
| Full Name | dual specificity phosphatase 3 |
| Calculated molecular weight | 185 aa, 20 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC002682 |
| Gene Symbol | DUSP3 |
| Gene ID (NCBI) | 1845 |
| RRID | AB_2918154 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51452 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DUSP3 antibody 28284-1-AP | Download protocol |
| WB protocol for DUSP3 antibody 28284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Tissue Cell Targeting DUSP3 promotes cell senescence by activating the notch1 pathway to treat hepatocellular carcinoma
| ||
Int J Nanomedicine USC-Derived Small Extracellular Vesicles-Functionalized Scaffolds Promote Scarless Vaginal Defect Repair via Delivery of Decorin and DUSP3 Proteins | ||
Sci Rep Piezo1 activation protects against sepsis-induced myocardial dysfunction in a pilot study | ||
Immun Inflamm Dis Dehydroandrographolide facilitates M2 macrophage polarization by downregulating DUSP3 to inhibit sepsis-associated acute kidney injury |