Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21870-1-AP targets DUS4L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16145 Product name: Recombinant human DUS4L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-197 aa of BC111774 Sequence: AMAEGYGACLINKPELVQDMVKQVRNQVETPGFSVSIKIRIHDDLKRTVDLCQKAEATGVSWITVHGRTAEERHQPVHYDSIKIIKENMSIPVIANGDIRSLKEAENVWRITGTDGVMVARGLLANPAMFAGYEETPLKCIWDWVDIALELGTPYMCFHQHLMYMMEKITSRQEKRVFNALSSTSAIIDYLTDHYGI 相同性解析による交差性が予測される生物種 |
| Full Name | dihydrouridine synthase 4-like (S. cerevisiae) |
| Calculated molecular weight | 317 aa, 36 kDa |
| Observed molecular weight | 22 kDa, 30 kDa |
| GenBank accession number | BC111774 |
| Gene Symbol | DUS4L |
| Gene ID (NCBI) | 11062 |
| RRID | AB_2878933 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95620 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
tRNA-dihydrouridine synthase 4-like belongs to the dus family.It catalyzes the synthesis of dihydrouridine and a modified base found in the D-loop of most tRNAs.Researches showed that DUS4L was alsophosphorylated in vitro by a Ca2+ calmodulin-activated kinase. It has 2 isoforms produced by alternative splicing .One, at 35 kDa, comigrated with a second cAMP-stimulated RIS-ROS phosphoprotein,DUS4L,which was also detected in the cytoskeleton.This antibody recognize the C-terminal of DUS4L.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DUS4L antibody 21870-1-AP | Download protocol |
| WB protocol for DUS4L antibody 21870-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
