Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse colon tissue, Jurkat cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
26632-1-AP targets DUOXA1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24373 Product name: Recombinant human DUOXA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 162-298 aa of BC020841 Sequence: GYMLLATGIFQLLALLFFSMATSLTSPCPLHLGASVLHTHHGPAFWITLTTGLLCVLLGLAMAVAHRMQPHRLKAFFNQSVDEDPMLEWSPEEGGLLSPRYRSMADSPKSQDIPLSEASSTKAYCKEAHPKDPDCAL 相同性解析による交差性が予測される生物種 |
| Full Name | dual oxidase maturation factor 1 |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 29 kDa, 52 kDa |
| GenBank accession number | BC020841 |
| Gene Symbol | DUOXA1 |
| Gene ID (NCBI) | 90527 |
| RRID | AB_2880581 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q1HG43 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DUOXA1, also named as NIP and NUMBIP, originally identified as a Numb-interacting protein, was recently shown to function as a maturation factor for the dual oxidase 1(DUOX1). DUOXA1 transient overexpression affected the cell-cell adhesion by modulating the actin cytoskeleton, and sensitized cells to doxorubicin. DUOXA1 has some isoforms with MW 33 kDa, 38 kDa and 54 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DUOXA1 antibody 26632-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |