Validation Data Gallery
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Tested Applications
| Positive WB detected in | SGC-7901 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IP | See 3 publications below |
Product Information
26143-1-AP targets DUB3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23567 Product name: Recombinant human DUB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 374-530 aa of BC157073 Sequence: QKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ 相同性解析による交差性が予測される生物種 |
| Full Name | deubiquitinating enzyme 3 |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC157073 |
| Gene Symbol | DUB3 |
| Gene ID (NCBI) | 377630 |
| RRID | AB_2880401 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6R6M4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DUB3 antibody 26143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Sarmentosin promotes USP17 and regulates Nrf2-mediated mitophagy and cellular oxidative stress to alleviate APAP-induced acute liver failure. | ||
Front Pharmacol Lysophosphatidic Acid-Induced EGFR Transactivation Promotes Gastric Cancer Cell DNA Replication by Stabilizing Geminin in the S Phase. | ||
Breast Cancer Res Treat USP17L2-SIRT7 axis regulates DNA damage repair and chemoresistance in breast cancer cells
| ||
Oncogene C4orf19 inhibits colorectal cancer cell proliferation by competitively binding to Keap1 with TRIM25 via the USP17/Elk-1/CDK6 axis | ||
iScience DUB3 is a MAGEA3 deubiquitinase and a potential therapeutic target in hepatocellular carcinoma | ||
FASEB J Melatonin alleviates hyperoxia-induced lung injury through elevating MSC exosomal miR-18a-5p expression to repress PUM2 signaling |