Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 15360-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15360-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15360-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15360-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15360-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15360-1-AP (DTYMK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15360-1-AP
IP result of anti-DTYMK (IP:15360-1-AP, 3ug; Detection:15360-1-AP 1:1000) with HeLa cells lysate 2800ug.
IHC staining of human breast cancer using 15360-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15360-1-AP (DTYMK antibody) at dilution of 1:500 (under 10x lens).
IHC staining of human breast cancer using 15360-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15360-1-AP (DTYMK antibody) at dilution of 1:500 (under 40x lens).
IHC staining of human pancreas cancer using 15360-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15360-1-AP (DTYMK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 15360-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15360-1-AP (DTYMK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 15360-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15360-1-AP (DTYMK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 15360-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15360-1-AP (DTYMK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 15360-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15360-1-AP (DTYMK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 15360-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15360-1-AP (DTYMK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15360-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DTYMK antibody (15360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HepG2 using 15360-1-AP
Immunofluorescent analysis of HepG2 cells, using DTYMK antibody 15360-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

DTYMK Polyclonal antibody

DTYMK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15360-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TYMK, TMPK, EC:2.7.4.9, dTMP kinase, CDC8

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, human brain tissue, human heart tissue, mouse brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human pancreas cancer tissue, mouse kidney tissue, mouse small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
IHC	See 2 publications below

Product Information

15360-1-AP targets DTYMK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DTYMK fusion protein Ag7606 相同性解析による交差性が予測される生物種
Full Name	deoxythymidylate kinase (thymidylate kinase)
Calculated molecular weight	24 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC001827
Gene Symbol	DTYMK
Gene ID (NCBI)	1841
RRID	AB_2261817
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P23919
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DTYMK(Thymidylate kinase) is also named as CDC8,TMPK and TYMK.It catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis.This enzyme is important in cellular DNA synthesis because the synthesis of dTTP either via the de novo pathway or through the exogenous supply of thymidine requires the activity of this enzyme(PMID:18469).

Protocols

Product Specific Protocols
WB protocol for DTYMK antibody 15360-1-AP	Download protocol
IHC protocol for DTYMK antibody 15360-1-AP	Download protocol
IF protocol for DTYMK antibody 15360-1-AP	Download protocol
IP protocol for DTYMK antibody 15360-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IHC	Cancer Res Targeting p300/CBP attenuates hepatocellular carcinoma progression through epigenetic regulation of metabolism. Authors - Ling-Yan Cai KD Validated View Article
human	WB	Cells Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line. Authors - Madhu Kollareddy View Article
human	WB	Front Mol Biosci Deoxythymidylate Kinase as a Promising Marker for Predicting Prognosis and Immune Cell Infiltration of Pan-cancer. Authors - Tianfeng Lan View Article
human	WB	Proteomes Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins. Authors - Henrick Horita View Article
human	WB,IHC	Cell Death Dis Inhibition of DTYMK significantly restrains the growth of HCC and increases sensitivity to oxaliplatin. Authors - Fengze Sun KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 15360-1-AP

WB analysis of human heart using 15360-1-AP

WB analysis of human brain using 15360-1-AP

WB analysis of mouse brain using 15360-1-AP

WB analysis of HepG2 using 15360-1-AP

IP experiment of HeLa using 15360-1-AP

IHC staining of human breast cancer using 15360-1-AP

IHC staining of human breast cancer using 15360-1-AP

IHC staining of human pancreas cancer using 15360-1-AP

IHC staining of human pancreas cancer using 15360-1-AP

IHC staining of mouse small intestine using 15360-1-AP

IHC staining of mouse small intestine using 15360-1-AP

IHC staining of mouse kidney using 15360-1-AP

IHC staining of mouse kidney using 15360-1-AP

IF Staining of HepG2 using 15360-1-AP

IF Staining of HepG2 using 15360-1-AP

DTYMK Polyclonal antibody

DTYMK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting p300/CBP attenuates hepatocellular carcinoma progression through epigenetic regulation of metabolism.

Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.

Deoxythymidylate Kinase as a Promising Marker for Predicting Prognosis and Immune Cell Infiltration of Pan-cancer.

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Inhibition of DTYMK significantly restrains the growth of HCC and increases sensitivity to oxaliplatin.