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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DTX3L Recombinant antibody (85340-2-RR)

    WB analysis using 85340-2-RR (same clone as 85340-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85340-2-RR (DTX3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85340-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DTX3L Recombinant antibody (85340-2-RR)

    IHC staining of human ovary cancer using 85340-2-RR (same clone as 85340-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85340-2-RR (DTX3L antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85340-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using DTX3L Recombinant antibody (85340-2-RR)

    IF Staining of Jurkat using 85340-2-RR (same clone as 85340-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DTX3L antibody (85340-2-RR, Clone: 242627C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85340-2-PBS in a different storage buffer formulation.

DTX3L Recombinant monoclonal antibody, PBS Only

DTX3L Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85340-2-PBS
Clone No.242627C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242627C8, BBAP, B-lymphoma- and BAL-associated protein, deltex 3 like (Drosophila), E3 ubiquitin-protein ligase DTX3L

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85340-2-PBS targets DTX3L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2568

Product name: Recombinant human DTX3L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 430-741 aa of BC060509

Sequence: SVHAYASFIDAFQHASCQLMREVLLLKSLGKERKHLHQTKFADDFRKRHPNVHFVLNQESMTLTGLPNHLAKAKQYVLKGGGMSSLAGKKLKEGHETPMDIDSDDSKAASPPLKGSVSSEASELDKKEKGICVICMDTISNKKVLPKCKHEFCAPCINKAMSYKPICPTCQTSYGIQKGNQPEGSMVFTVSRDSLPGYESFGTIVITYSMKAGIQTEEHPNPGKRYPGIQRTAYLPDNKEGRKVLKLLYRAFDQKLIFTVGYSRVLGVSDVITWNDIHHKTSRFGGPEMYGYPDPSYLKRVKEELKAKGIE

相同性解析による交差性が予測される生物種
Full Name deltex 3-like (Drosophila)
Calculated molecular weight 740 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC060509
Gene Symbol DTX3L
Gene ID (NCBI) 151636
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8TDB6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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