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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DTNA Polyclonal antibody (10741-1-AP)

    WB analysis using 10741-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10741-1-AP (DTNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using DTNA Polyclonal antibody (10741-1-AP)

    IP experiment of mouse brain using 10741-1-AP

    IP result of anti-DTNA (IP:10741-1-AP, 4ug; Detection:10741-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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DTNA Polyclonal antibody

DTNA Polyclonal Antibody for WB, IP, ELISA
Cat No. 10741-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Alpha dystrobrevin, D18S892E, DRP3, DTN, DTN A, DTNA, Dystrobrevin alpha, dystrobrevin, alpha, Dystrophin related protein 3, LVNC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, rat heart
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10741-1-AP targets DTNA in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DTNA fusion protein Ag1075 相同性解析による交差性が予測される生物種
Full Name dystrobrevin, alpha
Calculated molecular weight 42 kDa
Observed molecular weight25, 49-59 kDa, 78-85 kDa
GenBank accession numberBC005300
Gene Symbol DTNA
Gene ID (NCBI) 1837
RRIDAB_2093895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4J8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DTNA (dystorbrevin alpha) is a cytoskeleton associated protein and an essential component of dystrophin protein complex. It is highly expressed in brain, heart and skeletal muscle. Alternative splicing generates several isoforms ranging from 22 to 84 kDa.

Protocols

Product Specific Protocols
WB protocol for DTNA antibody 10741-1-APDownload protocol
IP protocol for DTNA antibody 10741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols