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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DTL Recombinant antibody (83107-1-RR)

    IF Staining of HeLa using 83107-1-RR (same clone as 83107-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DTL antibody (83107-1-RR, Clone: 230144C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83107-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using DTL Recombinant antibody (83107-1-RR)

    FC experiment of HeLa using 83107-1-RR (same clone as 83107-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DTL Recombinant antibody (83107-1-RR, Clone:230144C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83107-1-PBS in a different storage buffer formulation.

DTL Recombinant monoclonal antibody, PBS Only

DTL Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83107-1-PBS
Clone No.230144C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Denticleless protein homolog, DDB1- and CUL4-associated factor 2, DCAF2, CDW1, CDT2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83107-1-PBS targets DTL in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag33391

Product name: Recombinant human DTL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC033540

Sequence: MLFNSVLRQPQLGVLRNGWSSQYPLQSLLTGYQCSGNDEHTSYGETGVPVPPFGCTFSSAPNMEHVLAVANEEGFVRLYNTESQSFRKKCFKEWMAHWNAVFDLAWVPGELKLVTAAGDQTAKFWDVKAGELIGTCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMVWDTRCNKKDGFYRQVNQISGAHNTSDKQTPSKPKKKQNSKGLAPSVDFQQSVTVVLFQDENTLVSAGAVDGII

相同性解析による交差性が予測される生物種
Full Name denticleless homolog (Drosophila)
Calculated molecular weight 730 aa, 79 kDa
GenBank accession numberBC033540
Gene Symbol DTL
Gene ID (NCBI) 51514
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NZJ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).