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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DSG2 Recombinant monoclonal antibody (87426-1-RR)

    WB analysis using 87426-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87426-1-RR (DSG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DSG2 Recombinant monoclonal antibody (87426-1-RR)

    IF Staining of A431 using 87426-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DSG2 antibody (87426-1-RR, Clone: 251405G8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DSG2 Recombinant monoclonal antibody (87426-1-RR)

    IF Staining of MCF-7 using 87426-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DSG2 antibody (87426-1-RR, Clone: 251405G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87426-1-RR

    Affinity of 87426-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87426-1-RR against Human DSG2 were performed. The affinity constant is 2.32 nM.

DSG2 Recombinant monoclonal antibody

DSG2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87426-1-RR
Clone No.251405G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, Desmoglein 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, A431 cells
Positive IF/ICC detected inA431 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87426-1-RR targets DSG2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6509

Product name: Recombinant human DSG2 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 50-609 aa of NM_001943.4

Sequence: AWITAPVALREGEDLSKKNPIAKIHSDLAEERGLKITYKYTGKGITEPPFGIFVFNKDTGELNVTSILDREETPFFLLTGYALDARGNNVEKPLELRIKVLDINDNEPVFTQDVFVGSVEELSAAHTLVMKINATDADEPNTLNSKISYRIVSLEPAYPPVFYLNKDTGEIYTTSVTLDREEHSSYTLTVEARDGNGEVTDKPVKQAQVQIRILDVNDNIPVVENKVLEGMVEENQVNVEVTRIKVFDADEIGSDNWLANFTFASGNEGGYFHIETDAQTNEGIVTLIKEVDYEEMKNLDFSVIVANKAAFHKSIRSKYKPTPIPIKVKVKNVKEGIHFKSSVISIYVSESMDRSSKGQIIGNFQAFDEDTGLPAHARYVKLEDRDNWISVDSVTSEIKLAKLPDFESRYVQNGTYTVKIVAISEDYPRKTITGTVLINVEDINDNCPTLIEPVQTICHDAEYVNVTAEDLDGHPNSGPFSFSVIDKPPGMAEKWKIARQESTSVLLQQSEKKLGRSEIQFLISDNQGFSCPEKQVLTLTVCECLHGSGCREAQHDSYVG

相同性解析による交差性が予測される生物種
Full Name desmoglein 2
Calculated molecular weight122 kDa
Observed molecular weight150 kDa
GenBank accession numberNM_001943.4
Gene Symbol DSG2
Gene ID (NCBI) 1829
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).

Protocols

Product Specific Protocols
IF protocol for DSG2 antibody 87426-1-RRDownload protocol
WB protocol for DSG2 antibody 87426-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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