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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DSG2 Polyclonal antibody (29206-1-AP)

    WB analysis using 29206-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 29206-1-AP (DSG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DSG2 Polyclonal antibody (29206-1-AP)

    WB analysis of HeLa using 29206-1-AP

    WB result of DSG2 antibody (29206-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DSG2 transfected HeLa cells.

  • Western Blot (WB) analysis of HEK-293 cells using DSG2 Polyclonal antibody (29206-1-AP)

    WB analysis of HEK-293 using 29206-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 29206-1-AP (DSG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DSG2 Polyclonal antibody (29206-1-AP)

    IF Staining of A431 using 29206-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DSG2 antibody (29206-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DSG2 Polyclonal antibody (29206-1-AP)

    IF Staining of A431 using 29206-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DSG2 antibody (29206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

DSG2 Polyclonal antibody

DSG2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29206-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, Desmoglein 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29206-1-AP targets DSG2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20633

Product name: Recombinant human DSG2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 345-608 aa of BC099655

Sequence: MKNLDFSVIVANKAAFHKSIRSKYKPTPIPIKVKVKNVKEGIHFKSSVISIYVSESMDRSSKGQIIGNFQAFDEDTGLPAHARYVKLEDRDNWISVDSVTSEIKLAKLPDFESRYVQNGTYTVKIVAISEDYPRKTITGTVLINVEDINDNCPTLIEPVQTICHDAEYVNVTAEDLDGHPNSGPFSFSVIDKPPGMAEKWKIARQESTSVLLQQSEKKLGRSEIQFLISDNQGFSCPEKQVLTLTVCECLHGSGCREAQHDSYV

相同性解析による交差性が予測される生物種
Full Name desmoglein 2
Calculated molecular weight 1118 aa, 122 kDa
Observed molecular weight150-160 kDa
GenBank accession numberBC099655
Gene Symbol DSG2
Gene ID (NCBI) 1829
RRIDAB_2918248
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for DSG2 antibody 29206-1-APDownload protocol
WB protocol for DSG2 antibody 29206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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