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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DRD5 Polyclonal antibody (20310-1-AP)

    WB analysis using 20310-1-AP (same clone as 20310-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20310-1-AP (DRD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using DRD5 Polyclonal antibody (20310-1-AP)

    WB analysis of mouse brain using 20310-1-AP (same clone as 20310-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20310-1-AP (DRD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DRD5 Polyclonal antibody (20310-1-AP)

    IHC staining of mouse brain using 20310-1-AP (same clone as 20310-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20310-1-AP (DRD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DRD5 Polyclonal antibody (20310-1-AP)

    IHC staining of mouse brain using 20310-1-AP (same clone as 20310-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20310-1-AP (DRD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using DRD5 Polyclonal antibody (20310-1-AP)

    FC experiment of SH-SY5Y using 20310-1-AP (same clone as 20310-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug DRD5 Polyclonal antibody (20310-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20310-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DRD5 Polyclonal antibody, PBS Only

DRD5 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 20310-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20310-1-PBS targets DRD5 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14145

Product name: Recombinant human DRD5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 351-477 aa of BC009748

Sequence: NSSLNPVIYAFNADFQKVFAQLLGCSHFCSRTPVETVNISNELISYNQDIVFHKEIAAAYIHMMPNAVTPGNREVDNDEEEGPFDRMFQIYQTSPDGDPVAESVWELDCEGEISLDKITPFTPNGFH

相同性解析による交差性が予測される生物種
Full Name dopamine receptor D5
Calculated molecular weight 477 aa, 53 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberBC009748
Gene Symbol DRD5
Gene ID (NCBI) 1816
RRIDAB_10699880
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP21918
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DRD5, is a G-protein coupled receptor and involved in diversified physiological and pathological processes. this receptor stimulates adenylyl cyclase activity and is expressed in the limbic area of the mammalian brain(PMID:14699430). Studies have shown that DRD5 is highly expressed in colonic macrophages and DRD5 deficiency exacerbates DSS(dextran sodium sulfate)-induced colitis(PMID:34001860). in addition, DRD5 agonists were potent inhibitors of pituitary tumor growth(PMID:28613975).

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