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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DRD2 Polyclonal antibody (55084-1-AP)

    WB analysis using 55084-1-AP (same clone as 55084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55084-1-AP (DRD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DRD2 Polyclonal antibody (55084-1-AP)

    WB analysis using 55084-1-AP (same clone as 55084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55084-1-AP (DRD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using DRD2 Polyclonal antibody (55084-1-AP)

    IP experiment of SH-SY5Y using 55084-1-AP (same clone as 55084-1-PBS)

    IP result of anti-DRD2 (IP:55084-1-AP, 4ug; Detection:55084-1-AP 1:300) with SH-SY5Y cells lysate 3000ug. This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DRD2 Polyclonal antibody (55084-1-AP)

    IF Staining of mouse brain using 55084-1-AP (same clone as 55084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DRD2 Polyclonal antibody (55084-1-AP)

    IF Staining of mouse brain using 55084-1-AP (same clone as 55084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DRD2 Polyclonal antibody (55084-1-AP)

    IF Staining of mouse brain using 55084-1-AP (same clone as 55084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using DRD2 Polyclonal antibody (55084-1-AP)

    IF Staining of mouse brain using 55084-1-AP (same clone as 55084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 55084-1-AP (DRD2 antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55084-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DRD2 Polyclonal antibody, PBS Only

DRD2 Polyclonal Antibody for WB, IF-P, IF-Fro, IP, Indirect ELISA
Cat No. 55084-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, IF-Fro, IP, Indirect ELISA

Synonyms

D(2) dopamine receptor, D2 dopamine receptor, D2DR, D2R, Dopamine D2 receptor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55084-1-PBS targets DRD2 in WB, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name dopamine receptor D2
Calculated molecular weight 51 kDa
Observed molecular weight50-60 kDa
GenBank accession numberNM_016574
Gene Symbol DRD2
Gene ID (NCBI) 1813
RRIDAB_10859941
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP14416
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.

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