Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat liver tissue, L02 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
27662-1-AP targets DPYD in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26599 Product name: Recombinant human DPYD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-99 aa of BC008379 Sequence: VLSKDSADIESILALNPRTQTHATLCSTSAKKLDKKHWKRNPDKNCFNCEKLENNFDDIKHTTLGERGALREAMRCLKCADAPCQKSCPTNLDIKS 相同性解析による交差性が予測される生物種 |
| Full Name | dihydropyrimidine dehydrogenase |
| Calculated molecular weight | 1025 aa, 111 kDa |
| Observed molecular weight | 111 kDa |
| GenBank accession number | BC008379 |
| Gene Symbol | DPYD |
| Gene ID (NCBI) | 1806 |
| RRID | AB_2880941 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12882 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DPYD antibody 27662-1-AP | Download protocol |
| WB protocol for DPYD antibody 27662-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Pharmgenomics Pers Med Exploration and Validation of Pancreatic Cancer Hub Genes Based on Weighted Gene Co-Expression Network Analysis and Immune Infiltration Score Analysis | ||
J Cell Mol Med Pathway-based stratification of gliomas uncovers four subtypes with different TME characteristics and prognosis | ||
Theranostics Single cell analyses reveal the PD-1 blockade response-related immune features in hepatocellular carcinoma | ||
J Clin Invest CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes | ||
Nat Commun Proteomic profiling of gliomas unveils immune and metabolism-driven subtypes with implications for anti-nucleotide metabolism therapy | ||
Clin Transl Oncol Pyrimidine metabolism reshapes immune microenvironment and implies poor prognosis in glioma
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