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Validation Data Gallery

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  • Western Blot (WB) analysis of rat skin tissue using Dermatopontin Polyclonal antibody (14777-1-AP)

    WB analysis of rat skin using 14777-1-AP

    rat skin tissue were subjected to SDS PAGE followed by western blot with 14777-1-AP (Dermatopontin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skin tissue using Dermatopontin Polyclonal antibody (14777-1-AP)

    WB analysis of rat skin using 14777-1-AP

    rat skin tissue were subjected to SDS PAGE followed by western blot with 14777-1-AP (Dermatopontin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Dermatopontin Polyclonal antibody

Dermatopontin Polyclonal Antibody for WB, ELISA
Cat No. 14777-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

DPT, Tyrosine-rich acidic matrix protein, TRAMP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inrat skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14777-1-AP targets Dermatopontin in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6200

Product name: Recombinant human DPT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-201 aa of BC033736

Sequence: MDLSLLWVLLPLVTMAWGQYGDYGYPYQQYHDYSDDGWVNLNRQGFSYQCPQGQVIVAVRSIFSKKEGSDRQWNYACMPTPQSLGEPTECWWEEINRAGMEWYQTCSNNGLVAGFQSRYFESVLDREWQFYCCRYSKRCPYSCWLTIEYPGHYGEEMDMISYNYDYYIRGATTTFSAVERDRQWKFIMCRMTEYDCEFANV

相同性解析による交差性が予測される生物種
Full Name dermatopontin
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC033736
Gene Symbol DPT/TRAMP
Gene ID (NCBI) 1805
RRIDAB_2878078
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07507
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DPT (dermatopontin) is an extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly and wound healing.

Protocols

Product Specific Protocols
WB protocol for Dermatopontin antibody 14777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols